Journal: Oncology Reports

Article Title: Exploring the role of disulfidptosis-related signatures in immune microenvironment, prognosis and therapeutic strategies of cholangiocarcinoma

doi: 10.3892/or.2026.9042

Figure Lengend Snippet: F-actin and nuclei in HuCCT1 and RBE cells transfected with si-NC, si-CD109 or si-EFNB2 and treated with 5 µM BAY-876 or 1 µM TCEP for 24 h. si, small interfering; NC, negative control; TCEP, Tris-(2-carboxyethyl)-phosphine hydrochloride.

Article Snippet: Cells were then treated with the GLUT1 inhibitor BAY-876 (10 μM; cat. no. HY-100017, MedChemExpress) and/or the reducing agent, Tris-(2-carboxyethyl)-phosphine hydrochloride (TCEP; 20 mM; cat. no. A600974; Sangon Biotech Co., Ltd.) for 6 h at 37°C.

Techniques: Transfection, Negative Control

Journal: Microbiology Spectrum

Article Title: D-glucose uptake inhibits bovine alphaherpesvirus 1 post-binding cell process entry via inhibition of PLC-γ1 signaling in a glucose transporter 1-independent manner

doi: 10.1128/spectrum.02456-25

Figure Lengend Snippet: Immunofluorescence detection of GLUT1 protein co-localization with the ER in cells infected with BoAHV-1. ( A ) MDBK cells were either mock-infected or infected with BoAHV-1 (MOI=1) for 8 and 24 h. ( B ) Neuro-2A cells were mock-infected or infected with BoAHV-1 (MOI=1) for 48 h. Then the ER of the cells was fluorescently labeled (red), and GLUT1 protein was immunostained via IFA assay using GLUT1-specific antibody (green). The nuclei were stained using DAPI (blue). Images were captured using a confocal microscope. The data shown are representations of three independent experiments. Scale bar: 10 μm.

Article Snippet: Phospholipase C (PLC) inhibitor U73122 (cat# HY-13419) and GLUT1-specific inhibitor BAY-876 (cat# HY-100017) were ordered from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Immunofluorescence, Infection, Labeling, Staining, Microscopy

Journal: Microbiology Spectrum

Article Title: D-glucose uptake inhibits bovine alphaherpesvirus 1 post-binding cell process entry via inhibition of PLC-γ1 signaling in a glucose transporter 1-independent manner

doi: 10.1128/spectrum.02456-25

Figure Lengend Snippet: Detection of GLUT1 and virion-associated proteins in BoAHV-1 infected MDBK and Neuro-2A cells using IFA. MDBK (A, C, and D) and Neuro-2A cells were either mock-infected or infected with BoAHV-1 at an MOI of 1 for different durations under designated conditions. Specifically, MDBK cells were infected for 24 h at 37°C to allow for virus productive infection ( A ), for 1 h at 4°C to allow for virus binding to the cells ( C ), or for 1 h at 37°C to allow for virus binding and subsequent entry ( D ). Neuro-2A cells were infected for 24 and 72 h at 37°C ( B ). Then these cells were subjected to immunostaining using GLUT1-specific antibody (red) and anti-BoAHV-1 serum (green). The cell nuclei were stained with DAPI (blue). Images were then captured using a confocal microscope. Scale bars: 20 μm.

Article Snippet: Phospholipase C (PLC) inhibitor U73122 (cat# HY-13419) and GLUT1-specific inhibitor BAY-876 (cat# HY-100017) were ordered from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Infection, Virus, Binding Assay, Immunostaining, Staining, Microscopy